Fascination About thr77 login
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in lots of tumors, which leads to chromosome instability, and therefore may well lead to tumorigenesis. This protein was uncovered to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a vital part in promoting cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been described. Two more splice variants were being noted but specific nucleotide sequence info just isn't however offered.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide typically contains the epitope regarded because of the antibody.
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This guideline outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best benefits are...
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This manual outlines even further possible optimization of cellular and immunoassay parameters to guarantee the best possible final results are...
Antibodies sure to the blocking peptide now not bind to the epitope about the concentrate on protein. By comparing the staining from your blocked antibody as opposed to the antibody by yourself, you can see which staining is precise.
Concentrate on Information and facts Cyclin E belongs to the extremely conserved cyclin household, whose associates are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which lead to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S section boundary and is also degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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